Qubit Systems produces the only complete experimental package Q-Box NF1LP for measurement of nitrogen fixation in H2 producing legume symbioses. The unique flow-through H2 sensor (Q-S121) measures the production rate of H2 from N2-fixing tissues, allowing in vivo measurement of nitrogenase activity in real time. When combined with Qubit Gas Switching System measurements of nitrogenase activity on up to 3 plants is possible (4 channel system including reference)
Measurement of H2 evolution as a means of determining nitrogenase activity overcomes all the problems of the traditional acetylene reduction assay. The Q-Box NF1LP Nitrogen Fixation Package is perfectly safe, non-invasive and allows changes in nitrogenase activity to be observed as they occur.
The Nitrogen Fixation Package contains everything required to conduct numerous laboratory investigations into the physiology and biochemistry of N2 fixation in an open-flow gas exchange system. We even supply legume seeds and innoculum! Calibration of analyzers is simple, set-up time is minimal and experiments are easy to perform. This system is not designed for field use
Features
A flow through gas analysis system for measurements in H2 evolving symbioses
Non-invasive measurements of nitrogenase activity from nodulated roots
Package includes gas exchange growth pots, soybean seeds and nodule innoculum
Applications
Study Effects of Temperature on Nitrogenase Activity
Study Oxygen Regulation of N2 Fixation
Studies of Electron Allocation Coefficient of Nitrogenase
Study N2 Fixation and Photosynthate Supply
Study Inhibition of N2 Fixation by Fertilizer Supply
Components
Q-S121 Hydrogen Analyzer (0-100ppm) includes drying column
Q-S102 O2 Analyzer 0-100%
C301 Event Switch (Air/Ar)
S132 Temperature Sensor
Q-G267 Pump & Flow Monitor (1L/min)
Q-G268 Pump & Flow Monitor (2L/min)
G122 Large Gas Bags (2)
G132 Plant Growth Pots (4) with lids, fittings and Qubitac
G161 Nodule Cuvette
B201 Soybean seeds with
Bradyrhizobium japonicum inoculant
C610 LabQuest mini (3) data interface system
C901 Logger Pro software
C404 Customized Setup Software
Rugged water-proof case housing all analyzers and sensors
Q-Box Accessory kit
Manual
Optional Components:
Gas Switching System (for up to 4 channels)
References
WangX et al (2018) Coupling between nitrogen fixation andtetrachlorobiphenyl dechlorination in a rhizobium–legume symbiosis.Environ Sci Technol 52: 22178-2224
BulgarelliRG et al (2017) Mycorrhizae enhance nitrogen fixation andphotosynthesis in phosphorus-starved soybean (Glycine max L. Merrill). Environmental andExperimental Botany 140: 26-33
FerreiraC et al (2016) pH effects on nodulation and biological nitrogenfixation in Calopogoniummucunoides Brazilian Journal of Botany 39, 1015–1020
KassawT, Bridges W, Frugoli J (2015) Multiple Autoregulation ofNodulation (AON) Signals Identified through Split Root Analysis ofMedicago truncatula sunn and rdn1 Mutants. Plants 4(2), 209-224;doi:10.3390/plants4020209
CabezaR et al (2014) An RNA sequencing transcriptome analysis revealsnovel insights into molecular aspects of the nitrate impact on noduleactivity of Medicagotruncatula. Plant Physiology 164:400-411 DOI:
Justino GC, Sodek L (2013)Recovery of nitrogen fixation after short-term flooding of the nodulatedroot system of soybean. Journal of Plant Physiology 170:235-241
